Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

Marchetti, Vittoria Mattioni and Bitar, Ibrahim and Sarti, Mario and Fogato, Elena and Scaltriti, Erika and Bracchi, Chiara and Hrabak, Jaroslav and Pongolini, Stefano and Migliavacca, Roberta (2021) Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy. Diagnostics, 11 (1). p. 79. ISSN 2075-4418

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Abstract

Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy. Methods: species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the blaVIM- and mcr- type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio). Results: the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The mcr-9.2 gene was harbored on a conjugative IncHI2 plasmid, while the blaVIM-1 determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: mcr-9 gene and its two component regulatory genes for increased expression on the chromosome, mcr-4.3 on non-conjugative (yet co-transferable) ColE plasmid, and blaVIM-1 on a non-conjugative IncA plasmid. Conclusions: to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

Item Type: Article
Subjects: Library Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 29 Nov 2022 04:54
Last Modified: 13 Jul 2024 13:27
URI: http://news.pacificarchive.com/id/eprint/485

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